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Abad-Garcia, B., Berrueta, L. A., Garmon-Lobato, S., Gallo, B., & Vicente, F. (2009).

A sequential solvent extraction scheme was employed for the extraction of antioxidant compounds from kenaf (Hibiscus cannabinus L.) seeds. The diluted LDL cholesterol (0.5 mL) was mixed with 100 μL of the sample solution. LC flow was analyzed online by a mass selective detector system (LC-MSD-Trap-SL, Agilent) in electrospray ionization (ESI) mode. 6C).

In all antioxidant tests, the dechlorophyllized soluble phenolics extract from Medina mint had a higher antioxidant activity than the sample that was not dechlorophyllized (Table 1).
Found insideAssay Reference Biological activity Antioxidant Preethia et al., 2006 NBT reduction method TBARS assay DPPH assay ABTS assay Griess assay In vivo enzymatic antioxidant activity in mice (SOD, CAT, GP) BrdU kit Sulforhodamine B assay ...

The internal wells of a non-transparent 96-well microplate (Costar Corning Inc., Corning NY, USA) were used in the ORAC assay.

Pawlak et al.

(26) purified and isolated kaempferol as the active compounds of the stems of P. japonicus.

However, there has been no review (to our knowledge) that focuses exclusively on this test.

The phenolic composition was determined by HPLC-DAD.

2009) (Table 4).

The amount of TBARS formed in each of the samples was assessed by measuring optical density of the supernatant at 535 nm using spectrophotometer against a reagent blank.

J Clin Pathol. Plasma membranes senile dystrophic neurites appear more sensitive to β-amyloid deposition and lipid peroxidation processes that the membranes of normal cells or other neurites of neuritic plaques.

The mRNA amounts were normalized to an internal control, TATA-binding protein (TBP) mRNA. CRC Press, Taylor & Francis Inc., Boca Raton, FL.

2021 Sep 17;100(12):101493. doi: 10.1016/j.psj.2021.101493. Physicochemical properties of and volatile compounds in riboflavin fortified cloudy apple juice; study of its effect on job fatigue among Egyptian construction workers.

For example, after 3 to 5 days of storage, hexanal, a major breakdown product of linoleic acid, begins to decline perhaps due to its further oxidation or interaction with other meat components (Shahidi and Alexander 1998). 2001 May;54(5):339. doi: 10.1136/jcp.54.5.339. 2005). The antioxidant activity of both the extracts of the plant was measured with 1,1-diphenyl 2-picryl hydrazyl radical (DPPH) spectrophotometrically at 517 nm (Blois 1958). (1997).

The TaqMan gene expression assay for prostaglandin-endoperoxide synthase 2 (COX-2) was acquired from Applied Biosystems (Carlsbad, CA, USA) and used for gene detection. Assay: TBARS in catfish liver cells. J Agric Food Chem, 59, 9563–9571.

The DNA was suspended in a buffer solution containing a mixture of Tris base, acetic acid and EDTA (TAE buffer; pH 8.5) at a concentration of 25 μg/mL. As renal clearance and hemodialysis could alter plasma levels of free isoprostanes, the esterified F2-isoprostanes would be a more reliable marker of oxidative stress in this clinical setting [35].

There was a clear relationship between the total phenolic content and the antioxidant activity; samples with the higher phenolic content were more effective antioxidants, especially in the tests that measured free radical scavenging.

The obtained values of standards are plotted as a function of the known MDA concentration (expressed in μmol/mg protein units) and the results are calculated from the standard curve equation.

[33] have shown a significant decrease in total GSH content in plasma and in other localizations such as total blood and erythrocytes, without gender differences. This translational work provides that understanding. The book begins by covering the basic biology of oxidative stress from molecular biology to imaging in relation to diabetes.

The iron chelating activity assay measures the sample’s capacity to chelate ferrous ion and, since the TBARS assay measures secondary oxidation products, it indicates the total antioxidant activity.

2.2.

Inhibitory effect of mint extracts on LPS stimulated COX-2 expression in J774A.1 mouse macrophage cells.

For Hasawi mint, the total phenolic content was 36.80 and 1.54 mg GAE/g sample for the soluble and bound fractions, respectively. Indian Journal of Novel Drug Delivery, 11(1), 30-39.
The linear DNA band on the electrophoresis gel indicates the presence of double strand scission in the bound and aqueous samples, as well as the blank.

eCollection 2021 Oct. Dkhil MA, Abdel-Gaber R, Alojayri G, Thagfan FA, Al-Shaebi EM, Al-Quraishy S. Saudi J Biol Sci.

Chrysoeriol was absent from both dechlorophyllized samples but was present in both crude sample counterparts.

Found inside – Page 83Patatin has been shown to possess antioxidant activity (Wang and Xiong, 2005). ... of a potato protein hydrolysate fraction in cooked beef patties with the peroxide and thiobarbituric acid reactive substances (TBARS) antioxidant assays.

), methods Enzymol., 35: 190–203, Academic Press, San Diego, CA.

Found inside – Page 500Recent studies have pointed to an interaction between the bioactive compounds present in citrus fruits and antioxidant activity. Using the thiobarbituric acid reactive substances (TBARS) assay, Londofio-Londofio et al.

The J774A.1 cells were obtained from American Type Culture Collection (ATCC, Menassas VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum and 1% Amphotericin B/Streptomycin/Penicillin at 37 °C and 5% CO2. Nature of polyphenol-protein interactions. Major phenolic acids of local and exotic mint germplasm grown in Islamabad.

All of the compounds were found to yield [M-H]¯ ions of different masses, except caffeoylquinic acid and salvianic acid, which gave the corresponding [M-H + Na]− and [2 M-H]− ions, respectively. Here, we aimed to investigate the antioxidant and free radical scavenging properties of methanolic extracts from Tabebuia pallida (T. pallida) stem bark (TPSB . Specifically in plasma, some authors have described no changes in GSH content [42], although Cristalli et al.

Antioxidant activity by DPPH. Food Chem. TBARS method determines the extent of lipid peroxydation in sample. Authors contributed equality to the reported work. One of the compounds identified in both mint samples, carnosol, is not often reported as being present in mint; however, it is a major phenolic compound in rosemary, a relative of mint (Kayashima and Matsubara 2012).

ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Mint leaves of Mentha species were found to possess strong antioxidant properties in vitro by displaying free radical scavenging activity.

1 and 2.

Found inside – Page 131994), thiobarbituric acid‐reactive substances (TBARS) assay (Sinnhuber & Yu 1958), ultraviolet (UV) spectroscopic measurement of conjugated dienes (Gutteridge & Halliwell 1990), ferric thiocyanate and ferric xylenol orange assays ...

Catalase Colorimetric Activity Kit

2021 Aug 30;26:59. doi: 10.4103/jrms.JRMS_321_20. Tea and herbal infusions: their antioxidant activity and phenolic profile. However, no changes were observed between ACE1-D and ACE1-I. (4) Kim et al. A Comparative Study of Salivary Flow Rate, pH, Buffer Capacity, Total Antioxidant Capacity, and Ferritin Levels in Children with Beta Thalassemia Major and Healthy Children.

The molecular structure of lipids makes them susceptible to oxidation. Prevention and treatment information (HHS).

In other words, aqueous infusion of Medina mint had the lowest antioxidant activity in this assay and aqueous infusion of Hasawi mint had the highest activity. Negative control indicate COX-2 expression level without LPS stimulation and positive control was measured after 10 ng/mL LPS stimulation for 4 h. Cyclooxygenase-2 (COX-2)-catalysed synthesis of prostaglandin E2 plays a key role in inflammation and its associated diseases, such as cancer and cardiovascular disease (O’Leary et al. (1995).

These oils, together with 16-18 components found to be present, were tested for antioxidant properties at final concentrations of 0.05-2.5×10 4 ppm in an egg yolk‐based thiobarbituric acid reactive substances (TBARS) assay and also undiluted in a β‐carotene agar diffusion assay. Iron chelation activity for the purified phenolic extracts seems to be directly related to the total phenolic content; the values for Medina mint were still higher than those for Hasawi mint and the soluble phenolics extracts were still higher than the bound phenolics extracts. TBARS method determines the extent of lipid peroxydation in sample. The absorbance was read at 700 nm and the results were expressed as mmol TE/100 g of initial dried sample. The result is quantified with a calibration curve using MDA or in term of percentage inhibition.27, Guy Landau, ... Randal J. Kaufman, in Methods in Enzymology, 2013.

The mRNA expression of the inflammatory mediator, cyclooxygenase-2 (COX-2) was monitored in order to test the involvement of transcriptional events during their biosynthesis.

Elia Escasany, ... Gema Medina-Gómez, in Obesity, 2018. Am J Enol Viticult, 16, 144–158. Peak identities are numbered in Table 3.4, ESI-MS/MS spectra of peak no. The chloroform was evaporated from the lipids using a Rotavapor (Buchi, Flawil, Switzerland) while increasing heat and decreasing pressure until all the solvent was removed.

3 in Fig. F2-Isoprostane (8-iso-PGF2α, where PGF is prostaglandin) is produced by nonenzymatic oxidation of arachidonic acid by reactive oxygen species including free radicals. Optimized DPPH assay in a detergent-based buffer system for.

Correspondence to Leukotrienes and F2-isoprostane can also be used as markers of oxidative stress in breath condensate. Antioxidants can scavenge free radicals and minimize their impact.

For example, increased levels of TBARS have been observed in cardiac muscle of obese Zucker Fatty rats and in diet-induced obese Wistar rats, which were returned to normality by treatment with statins [126].

The soluble phenolics and aqueous infusion samples from Medina and Hasawi mint were diluted in 10 mM PBS to obtain a concentration of 0.1 mg extract per mL. In, Packer, L.

The effects of climate change on medicinal and aromatic plants. The antioxidant activities were determined by DPPH assay, lipid peroxidation assay, iron chelation and total antioxidant assay. The supercoiled plasmid DNA strand inhibition assay measures the effectiveness of the samples in preventing DNA strand scission by peroxyl radicals. Evaluation of antioxidants: scope, limitations and relevance of assays. In general, alkanes along with monomethylated alkanes are formed due to lipid peroxidation that can be used as markers of oxidative stress [41].

The chelation of ferrous ions by mint samples was estimated by comparison with ethylenediaminetetraacetic acid (EDTA) according to a modified version of the method described by Wu and Ng (2008).

Phytother Res, 24, 869–874. Oxidation of LDL cholesterol was initiated by adding 400 μL of 12.5 μM cupric sulphate solution in distilled water. Superoxide dismutase, TBARS, and GSH all correlated well with behavioral parameters39. Politeo, O., Jukic, M., & Milos, M. (2006). Briefly, 2.0 g of each meat mixture were blended with 5.0 mL of 10% (w/v) trichloroacetic acid (TCA).

Prior, R. L., Wu, X., & Schaich, K. (2005).

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